Tissue Distribution of Mevalonate Pyrophosphate Decarboxylase in Guinea Pig
نویسندگان
چکیده
منابع مشابه
Subcellular distribution of mouse mevalonate pyrophosphate decarboxylase.
Mevalonate pyrophosphate decarboxylase (MPD) is considered to be a cytosolic protein. Recently, other groups reported that MPD is mostly located in the peroxisomes. In this study, we examined whether the expression of MPD in mice depends on the proliferation of peroxisomes, and whether MPD is predominantly located in the peroxisomes or the cytosol of mice. No increase in the protein level of MP...
متن کاملPurification and characterization of mouse mevalonate pyrophosphate decarboxylase.
Mevalonate pyrophosphate decarboxylase (MPD) in mouse liver was purified by affinity chromatography. The purified enzyme was a homodimer of 46-kDa subunits and had an isoelectric point of 5.0. Kinetic analysis revealed an apparent Km value of 10 microm for mevalonate pyrophosphate. The enzyme required ATP as a phosphate acceptor and Mg as a divalent cation, which could be substituted with Mn or...
متن کاملInhibition of rat liver mevalonate pyrophosphate decarboxylase and mevalonate phosphate kinase by phenyl and phenolic compounds.
1. Mevalonate pyrophosphate decarboxylase of rat liver is inhibited by various phenyl and phenolic acids. 2. Some of the phenyl and phenolic acids also inhibited mevalonate phosphate kinase. 3. Compounds with the phenyl-vinyl structure were more effective. 4. Kinetic studies showed that some of the phenolic acids compete with the substrates, mevalonate 5-phosphate and mevalonate 5-pyrophosphate...
متن کاملTissue distribution of cholesterol feedback control in the guinea pig.
The level of cholesterol synthesis and the activity of the cholesterol feedback system were studied in tissue slices from a number of organs of the guinea pig. In contrast to the tissue distribution of sterol synthesis in the rat, liver slices of the guinea pig have a low rate of sterologenesis, with ileum and lung being the most active sterologenic tissues. More surprising, all tissues studied...
متن کاملComparison of subcellular distribution of mevalonate pyrophosphate decarboxylase between stroke-prone spontaneously hypertensive rat and Wistar Kyoto rat.
We previously reported that the lower activity of mevalonate pyrophosphate decarboxylase (MPD) was caused by the reduced amount of this enzyme in stroke-prone spontaneously hypertensive rat (SHRSP) by immunoblot analysis using 20,000 x g supernatant containing cytosol and microsomes. A recent study showed that at least three different subcellular compartments, including peroxisomes, are involve...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2009
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.32.1476